Thanks! I shot it at f22 and I have tried at f32, but I noticed quite a bit of contrast and sharpness loss (diffraction maybe?). Since the magnification is high, making the dof so thin, I could not get the whole droplet in focus. I was aiming for the refraction. There is way to do that by taking multiple pics of different sections of the subject, and using software to focus stack them into one picture with huge dof.